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KMID : 0385520080210060518
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Analytical Science & Technology
2008 Volume.21 No. 6 p.518 ~ p.525
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Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry
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Kwon Chan-Hyeok
Chang Moon-Ik
Im Moo-Hyeog
Choi Hoon
Jung Da-I
Lee Su-Chan
Yu Jin-Young
Lee Young-Deuk
Lee Jong-Ok
Hong Moo-Ki
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Abstract
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Mandipropamid is a new mandelamide-type fungicide to control foliar Oomycete pathogens in some vegetables. An analytical method was developed to determine mandipropamid residues in agricultural commodities using high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Mandipropamid was extracted with methanol from grape, tomato, green pepper, Chinese cabbage and potato samples. The extract was diluted with saturated sodium chloride solution and distilled water, and dichloromethane partition was followed to recover the mandipropamid from the aqueous phase. Florisil column chromatography was employed to further remove interfering co-extractives prior to HPLC analysis. Reverse-phased HPLC was successfully applied to determine mandipropamid in sample extracts with the detection at its λmax (223 ㎚). Overall recoveries of mandipropamid from fortified samples averaged 99.8±1.7 (n=6), 89.3±5.3 (n=6), 98.7±2.2 (n=6), 99.7±6.8 (n=6) and 91.1±3.1 (n=6) for grape, tomato, green pepper, Chinese cabbage and potato, respectively. Limit of quantification of the method was 0.02~0.04 ㎎/㎏ for all samples. A LC/mass spectrometry with selected-ion monitoring was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to determine the terminal residue of mandipropamid in agricultural commodities.
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KEYWORD
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mandipropamid, fungicide, residue, agricultural commodity
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